DMF treatment alters lipid metabolism in oligodendroglial cells. MO3. 13 cells were treated with DMF for 24 or 72 h and their lipids were analyzed by using shotgun lipidomics. (A) Lipid profile of MO3.13 cells after 24 h of DMF treatment. Decreased levels of PC (p<0.01) and SM (p<0.05) but increased PE (p<0.01), PI (p<0.01) species were detected. (B) Lipid profile of MO3.13 cells after 72 h of DMF treatment. Both PC (p<0.01) and SM (p<0.01) are significantly increased compared to vehicle controls. **p<0.01, *p-value <0.05, Kruskal–Wallis test, N=6 replicates per condition, repeated three times.