Fig. 5.

Epi-fluorescent images representing cellular death or apoptosis estimated by in situ cell death detection kit after 24 h treatment duration and apigenin mediated alterations in apoptotic and pro-apoptotic protein profile at high dosage treatment observed in colorectal cell lines HCT-15 and HT-29. (a) HCT-15 treated and untreated sets, (c) HT-29 treated and untreated sets. (A) Micrograph of control/untreated group, (B–H) micrograph of treated group with dosage starting from 1.5625 µM and ranging to 50 µM. (b and d) Mean fluorescence intensity of treated and untreated sets in HT-29 and HCT-15 respectively have been represented as fold increase with respect to control. (e) Western blot analysis of Bcl-2, Bax in colorectal cancer cells untreated/treated with high dose of apigenin. (f and g) Densitometric analysis of the protein bands obtained by western blot in apigenin treated/untreated HCT-15 and HT-29 cells respectively.