Figure 1.

Inhibitory effect of UPE on melanin content (A) and cell viability (B) of B16F10 cells. B16F10 cells (2.0×10⁴ µg/mL) were pre-incubated for 18 h and the melanin content was assayed after incubation of the B16F10 cells treated with α-MSH (100 nM), melasolv (40 µM), and UPE (3.125, 6.25 and 12.5 µg/mL) for 72 h at 37°C in a 5% CO₂ atmosphere. The absorbance was measured at 405 nm by ELISA. MTT assay was performed after incubation of the B16F10 cells treated with varying concentrations of UPE (3.125, 6.25 and 12.5 µg/mL) for 24 h at 37°C in a 5% CO₂ atmosphere. The absorbance was measured at 570 nm with a spectrophotometer (Power Wave; Bio-tek, Winooski, VT). Values are the mean ± SEM of triplicate experiments. *p<0.05; **p<0.01.