Figure 2.

Inhibitory effect on tyrosinase activity of UPE in B16F10 cells. B16F10 cells (2.0×10⁴ µg/mL) were pre-incubated for 18 h and tyrosinase activity was assessed after incubation of B16F10 cells treated with α-MSH (100 nM), melasolv (40 uM) and UPE (3.125, 6.25 and 12.5 µg/mL) for 72 h at 37°C in a 5% CO₂ atmosphere. Absorbance was measured at 405 nm with a ELISA. Values are the mean ± SEM of triplicate experiments.*p<0.05; **p<0.01.