Fig. 4.

Association of PlGF-mediated enhancement of SCs motility with increased phosphorylation of ERK1/2. aSCs were maintained for 0 hr and 24 hr with 4 nM PlGF and FBS-free DMEM after being scratched. Untreated aSCs (control) were incubated with FBS-free DMEM for 0 hr and 24 hr after being scratched, and cell motility of aSCs maintained with PlGF was analyzed (a). aSCs in FBS-free DMEM were treated with 4 nM PlGF for indicated time points, and total ERK (t-ERK), total AKT (t-AKT) and phosphorylated forms of ERK (p-ERK) and AKT (p-AKT) were analyzed by western blotting. β-actin was used as a loading control (b). All experiments were repeated at least three times. Scale bar in micrograph, 500 µm (*P<0.05).