Fig. 5.

Involvement of the ERK and PI3K pathways in the enhanced motility of aSCs. aSCs were incubated with 30 µM U0126 for 0 hr and 24 hr after being scratched. Untreated aSCs incubated with FBS-free DMEM for 0 hr and 24 hr after being scratched were used as a control. Cell motility of aSCs maintained with different treatments was analyzed (a). aSCs in FBS-free DMEM were treated with 30 µM of U0126 for indicated time points and then assessed for total and phosphorylated levels of ERK1/2 and AKT by western blotting (b). aSCs were incubated with 10 µM LY294002 in FBS-free medium or both U0126 and LY294002 for 0 hr and 24 hr after being scratched. Untreated aSCs incubated with FBS-free DMEM for 0 hr and 24 hr after being scratched were used as a control (c). All experiments were repeated at least three times. Scale bar, 500 µm (*P<0.05).