Detection of bovine ephemeral fever virus gene by RT-PCR assay. The specificity of
our RT-PCR assay was checked with RNA templates extracted from bovine ephemeral fever
virus and other viruses. Products of the RT-PCR assay separated on agarose gels and
stained with ethidium bromide are shown. Lanes 1 to 19, bovine ephemeral fever virus
(lane 1, ON-3/E/12; lane 2, ON-1/B/04; lane 3, ON-BEF-01-1; lane 4, ON-BEF-01-2; lane
5, ON-BEF-01-3; lane 6, ON-BEF-89-1; lane 7, ON-BEF-89-2; lane 8, ON-BEF-89-3; lane 9,
Onna 3; lane 10, ON-BEF-88-1; lane 11, ON-BEF-88-3; lane 12, ON-BEF-88-4; lane 13,
Hirado-6; lane 14, Hirado-9; lane 15, Amakusa-1; lane 16, Amakusa-2; lane 17, Azuma;
lane 18, YHL; lane 19, BB7721); lane 20 Akabane virus KM-1/Br/06; lane 21, Aino virus
KS-1/E/02; lane 22, Peaton virus ON-10/E/01; lane 23, Chuzan (Kasba) virus 31; lane
24, Ibaraki virus No.2; lane 25, bluetongue virus TO2-1; lane 26, negative control
(RNase-free water). M, molecular mass ladder (100 bp).