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Detection of bovine ephemeral fever virus gene in RNA extracted from field-collected bovine PBMCs. Two groups of RNA samples, Group A (lanes 1 to 6) and Group B (lanes 7 to 11), were used as templates for the RT-PCR assay. Products of the RT-PCR assay separated on agarose gels and stained with ethidium bromide are shown. M, molecular mass ladder (100 bp); lane 12, positive control (BEFV YHL); lane 13, negative control (RNase-free water).
