Table 1. Oligonucleotide primers used for RT-PCR and sequencing of bovine ephemeral fever virus.
| Primer | Sequence (5’–3’) | Position | Purpose | References |
|---|---|---|---|---|
| BEFV-AO-F | GAATCATTATGGGATCGGATC | 1140–1160 | RT-PCR | This study |
| BEFV-AO-R | CCAACCTACAACAGCAGATAAAAC | 1587–1564 | ||
| EG2 | TACAACAGCAGATAAAAC | 1581–1564 | RT-PCR | [8] |
| EG3 | CATTATGGGATAGGATCC | 1144–1161 | ||
| GF1 | ATGTTCAAGGTCCTCATAATTACC | 1–24 | RT-PCR, sequencing and cloning | [7] |
| G681F | ATGGGAGGCTCCAGATATCGGG | 681–702 | Sequencing | |
| G1414F | GAGGTAATGGAGTACGATAAC | 1414–1434 | Sequencing | |
| G1481F | AAGCCAGTGAATTTAAGCCC | 1480–1499 | Sequencing | |
| GR1 | TAATGATCAAAGAACCTATCATCA | 1871–1848 | RT-PCR, sequencing and cloning | |
| G1310R | GCTTCGTTCCGGAGCTTCCT | 1329–1290 | Sequencing | |
| G589R | CTCTCACATCTGGTATCCATGTCC | 589–575 | Sequencing | |
| G410R | TTCCAAAAGCATCCAGCAGGAGGG | 410–387 | Sequencing |