Table 2. Comparison of elution rates of vRNA from FTA® cards using different eluents.
| Eluent | Elution time | ||||
|---|---|---|---|---|---|
| 5 min | 15 min | 30 min | 60 min | 24 hr | |
| TE-buffer | + | + | ++ | ++ | ++ |
| Trizol | ± | ++ | ++ | + | + |
| Nuclease-free water | + | + | + | + | + |
| RNA Rapid Extraction Solution | ± | + | + | + | + |
| Buffer AVL | – | – | – | – | – |
When the intensity of the positive control band by RT-PCR with the primer pairs of RCHL-Nfull-F and RCHL-Nfull-R was set at 100%, the integrated optical density at the undetected (0%), 0–25%, 25–50% and 50–75% is represented as –, ±, + and ++ respectively.