Table 1. Results from histochemistry of mucus components in neoplastic cells.
| Stains | Mucus components | Cases | |||||
|---|---|---|---|---|---|---|---|
| No. 1 | No. 2 | No. 3 | No. 4 | No. 5 | No. 6 | ||
| PAS | Glycoproteins [neutral mucin and acidic glycoproteins (sulfomucin and sialomucin)] | +++ | +++ | +++ | +++ | +++ | +++ |
| PAS-D | Glycogen | + | + | ++ | + | + | ++ |
| AB pH 2.5 | Acidic glycoproteins (sulfomucin and sialomucin) | +++ | +++ | +++ | +++ | +++ | +++ |
| Acidic mucopolysaccharides (hyaluronic acid, chondroitin, heparin, keratan and dermatan) | |||||||
| AB pH 1.0 | Acidic glycoprotein (sulfomucin) | + | + | + | + | + | + |
| AB pH 2.5 after hyaluronidase treatment | Acidic mucopolysaccharide (hyaluronic acid) | ++ | ++ | ++ | ++ | ++ | ++ |
| CI | Acidic glycoprotein (sialomucin), acidic mucopolysaccharides (hyaluronic acid, chondroitin, heparin, keratan and dermatan) | + | + | + | + | + | + |
| HID-AB pH 2.5 | Acidic glycoprotein (sulfomucin) and acidic mucopolysaccharides | – | – | – | – | – | – |
| Mayer’s mucicarmine | Epithelial mucin and neutral mucopolysaccharides | +++ | ++ | ++ | ++ | ++ | +++ |
| TB pH 2.5 | Acidic mucopolysaccharides (chondroitin and mucoitinsulfuric acid) | – | – | – | – | – | – |
Staining rate: –, 0%; +, 1–30%; ++, 31–60%; +++, > 60%, PAS: periodic acid-Schiff, PAS-D: periodic acid-Schiff-diastase, AB: alcian blue, CI: colloidal iron, HID: high iron diamine, TB: toluidine blue.