Table 2. Characteristics of the primary antibodies used in immunohistochemistry.
| Antbodies | Clones | Dilutions | Pretreatments | Antibody suppliers |
|---|---|---|---|---|
| α1-antitrypsin | 1:1 | Cm | Nichirei, Tokyo, Japan | |
| Brachyury | D-10 | 1:100 | Ca | Santa Cruz Biotechnology, Santa Cruz, U.S.A. |
| CK AE1/AE3 | AE1/AE3 | 1:1 | PK | Nichirei Corp, Tokyo, Japan |
| CK7 | VO-TL-12/30 | 1:50 | PK | Dakopatts, Glostrup, Denmark |
| CK9 | ks9.7 | 1:10 | Cm | Progen, Heidelberg, German |
| CK13 | Ks13.1 | 1:10 | Cm | Dakopatts, Glostrup, Denmark |
| CK14 | LL002 | 1:20 | Cm | Biomeda, Foster City, U.S.A. |
| CK18 | RGE53 | 1:50 | Cm | Progen, Heidelberg, German |
| CK19 | BA17 | 1:1 | Cm | Thermo Fisher Scientific, U.S.A. |
| CK20 | Ks20.8 | 1:50 | PK | Dakopatts, Glostrup, Denmark |
| E-cadherin | 4A2C7 | 1:100 | Cm | Invitrogen, Camarillo, U.S.A. |
| EMA | E29 | 1:50 | Cm | Dakopatts, Glostrup, Denmark |
| MUC1 | Ma695 | 1:100 | Cm | Novocastra, Newcaslle, U.K. |
| MUC2 | Ccp58 | 1:100 | Cm | Novocastra, Newcaslle, U.K. |
| MUC5AC | CLH2 | 1:100 | Cm | Novocastra, Newcaslle, U.K. |
| NSE | BBS/NC/VI-H14 | 1:100 | Cm | Dakopatts, Glostrup, Denmark |
| PCNA | PC10 | 1:100 | Ca | Dakopatts, Glostrup, Denmark |
| S100 protein | 1:1000 | Cm | Dakopatts, Glostrup, Denmark | |
| Vimentin | V9 | 1:100 | Cm | Dakopatts, Glostrup, Denmark |
CK: cytokeratin, EMA: epithelial membrane antigen, NSE: neuron-specific enolase, PCNA: proliferating cell nuclear antigen, MUC: mucin core protein, Cm: citrate buffer pH 6.0 at 15 min in microwave, Ca: citrate buffer pH 6.0 at 15 min in autoclave, PK: protein kinase.