Table 3. Characteristics of the primary antibodies used in immunohistochemistry.
| Antigens | Cases | Reference cases | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. 1 | No. 2 | No. 3 | No. 4 | No. 5 | No. 6 | Ferret [4] | Ferret [10] | Ferret [18] | Ferret [32] | Cat [2] | Dog [7] | Rat [26] | |
| CK AE1/AE3 | +++ | +++ | +++ | +++ | +++ | +++ | 20/20 | 2/4 | 1/1 | 2/2* | 1/1 | 1/1 | 9/9* |
| EMA | +++ | ++ | +++ | ++ | +++ | ++ | NT | 1/4 | NT | NT | NT | NT | NT |
| NSE | +++ | +++ | +++ | +++ | ++ | ++ | 18/20 | NT | NT | 2/2 | NT | 0/1 | 9/9 |
| S100 protein | +++ | +++ | +++ | +++ | ++ | ++ | 15/20 | 3/4 | NT | 2/2 | 1/1 | 1/1 | 7/9 |
| Vimentin | +++ | +++ | +++ | +++ | +++ | +++ | 20/20 | NT | 1/1 | 2/2 | 1/1 | 1/1 | NT |
| α1-antitrypsin | +++ | +++ | ++ | ++ | +++ | ++ | NT | NT | NT | NT | NT | NT | NT |
| Brachyury | +++ | +++ | +++ | +++ | +++ | +++ | NT | NT | NT | NT | NT | NT | NT |
| CK7 | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| CK9 | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| CK13 | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| CK14 | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| CK18 | +++ | +++ | ++ | +++ | +++ | +++ | NT | NT | NT | NT | NT | NT | NT |
| CK19 | +++ | +++ | +++ | ++ | +++ | +++ | NT | NT | NT | NT | NT | NT | NT |
| CK20 | +++ | +++ | +++ | +++ | +++ | +++ | NT | NT | NT | NT | NT | NT | NT |
| E-cadherin | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| MUC1 | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| MUC2 | — | — | — | — | — | — | NT | NT | NT | NT | NT | NT | NT |
| MUC5AC | + | + | ++ | + | + | ++ | NT | NT | NT | NT | NT | NT | NT |
| PCNA | + | + | + | ++ | + | + | NT | NT | NT | NT | NT | NT | NT |
—: nonimmunoreactive; +: slightly immunoreactive; ++: moderately immunoreactive; +++: strongly immunoreactive, Number of immunoreactive animals/total number of animals, CK: cytokeratin; EMA: epithelial membrane antigen; NSE: neuron-specific enolase; CK: cytokeratin; MUC: mucin core protein; PCNA: proliferating cell nuclear antigen, *: keratin, [ ]: reference number, NT: not tested.