Table 1.

Primers used for PCR amplification of resistance genes.

Primer	5′-3′ sequence	Gene target	Amplicon size (bp)	TA	Reference
aadA1-F	TTTGATCAACGACCTTTTGGAAAC	aadA1 and aadA2	294	58°C	[9]
aadA-R	GGACAACGTAAGCACTACATTTCG
blaoxa30-F	ATTATCTACAGCAGCGCCAGTGCATC	bla OXA-30	716	63°C	This study
blaoxa30-R	TTCGACCCCAAGTTTCCTGTAAGTGC
blaPSE-1-F	GGATTACAATGGCAATCAGCGCTTCC	bla PSE-1	658	65°C	[10]
blaPSE-1-R	AATCGCATCATTTCGCTCTGCCATTG
blaTEM1-F	TGAAGATCAGTTGGGTGCACGAGTGG	bla TEM-1	700	63°C	[9]
blaTEM1-R	AGTTGCCTGACTCCCCGTCGTGTAGA
catA1-F	CACCGTTGATATATCCCAATGGCATCGT	catA1	582	62°C	This study
catA1-R	CTGCCGACATGGAAGCCATCACAAAC
dfrA1-F	TATTCCATGGAGTGCCAAAGGTGAAC	dfrA1	375	63°C	This study
dfrA1-R	GAGGCGAAGTCTTGGGTAAAAACTGG
dfrA12-F	CAGTACGCATTTATCTCGTTGCTGCG	dfrA12	417	63°C	This study
dfrA12-R	CAAGCTCGAATTCTGTTTCGTTGAGC
floR-F	CTCCTTTCGACATCCTCGCTTCACTG	floR	636	64°C	[10]
floR-R	AGAAGACGAAGAAGGTGCCCATACCG
sul1-F	TCGGCATTCTGAATCTCACCGAGGAC	sul1	786	64°C	[10]
sul1-R	AAATTTCGCGAGGGTTTCCGAGAAGG
sul2-F	GACAGTTTCTCCGATGGAGGCCGGTA	sul2	700	64°C	[10]
sul2-R	GTGTGCGGATGAAGTCAGCTCCACCT
strA-F	TGGTTGCCTGTCAGAGGCGGAGAATC	strAB	1466	63°C	[9]
strB-R	ATCGACGTCCAGCGCACGAGAGAATG
tetA-F	CCTGATCGTAATTCTGAGCACTGTCG	tet(A)	1089	63°C	This study
tetA-R	CACCCGTTCCACGTTGTTATAGAAGC
tetB-F	CAGGTTATCTTTGCTCCTTGGCTTGG	tet(B)	1014	63°C	[9]
tetB-R	TTGAGGGGTTAACATGAAGGTCATCG
tetC-F	GGATATCGTCCATTCCGACAGCATCG	tet(C)	745	63°C	[9]
tetC-R	GATAATGGCCTGCTTCTCGCCGAAAC
tetD-F	ACTGTCCAATGTGCTGTGGATGTTGT	tet(D)	747	63°C	This study
tetD-R	CTGACCAGCACACCCTGTAGTTTTCC
tetG-F	GAGCCGCAGTCGATTACACGATTATG	tet(G)	680	64°C	[10]
tetG-R	CAACAGAATCGGGAACACCATCCATC
intI1-F	CGAACCGAACAGGCTTATGTCCACTG	intI1	838	63°C	[10]
intI1-R	CATCGTCGTAGAGACGTCGGAATGG
5CS-F	GCCTCGGGCATCCAAGCAGCAAGC	5′CS attI1 end 3′CS	variable	65°C	This study
3CS-R	CTTGACCTGATAGTTTGGCTGTGAGCAA
U7-L12	ACACCTTGAGCAGGGCAAAG	tdhF	500	60°C	[4]
LJ-R1	AGTTCTAAAGGTTCGTAGTCG	int
104-RJ	TGACGAGCTGAAGCGAATTG	S044	515	60°C	[4]
C9-L	AGCAAGTGTGCGTAATTTGG	int2