Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 11;209(3):387–402. doi: 10.1083/jcb.201412144

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Lucena et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Imp1-mediated transport in the MMD region is importin-β–NLS cargo dependent. (A) Time-lapse fluorescence images of a cell coexpressing Imp1-Tom and Imp1-ΔIBB-ED-GFP. Only Imp1-Tom is found at the MMD. (B) Time-lapse fluorescence images of a cell expressing Imp1-ΔIBB-GFP. (graph) Mean maximal spindle length (percentage of the cell length) in wild-type and Imp1-ΔIBB cells (graph represents mean and standard deviation; n is the total number of cells scored from two independent experiments). (C) Time-lapse fluorescence images of Sid2-Tom in Imp1-ΔIBB cells. Arrows indicate the position of the SPB after cytokinesis is initiated. Kymograph of this cell is shown. Asterisk denotes a signal of a nearby cell. (D) MMD localization (arrows) of Kap95-GFP and Kap104-GFP importin-β proteins during anaphase B. (E) Time-lapse fluorescence images of a cell expressing Sal3-GFP importin-β protein. Kymograph of this cell is shown. Bars, 5 µm.