Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 11;209(3):387–402. doi: 10.1083/jcb.201412144

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Lucena et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Imp1-dependent levels of the spindle-stabilizing protein Ase1. (A) Western blot analysis of Ase1-GFP protein levels through the cell cycle in synchronized cdc25-22 and cdc25-22 imp1Δ cells. α-Tubulin is used as a control. Maximal septation index values are indicated. Kymographs of cdc25-22 and cdc25-22 imp1Δ cells expressing Ase1-GFP. Red arrow indicates midzone dissolution in cdc25-22 cells. Schematized cells (bottom) representing interphase, early anaphase, late anaphase B, ring contraction and daughter nuclei repositioning, and the end of cytokinesis, respectively, are shown. (B) Time-lapse fluorescence images of ase1Δ imp1Δ cells expressing Atb2-GFP and Sid2-Tom. Arrowhead indicates the initiation of spindle disassembly. (graph) Maximal spindle length in ase1Δ and ase1Δ imp1Δ cells, compared with wild-type and imp1Δ cells (from Fig. 1C). (C) Levels of Imp1-HA and Rpn8-GFP (Western blot analysis) in cells expressing Imp1-HA, Rpn8-GFP, or both tagged proteins in total extracts and in Imp1-HA immunopurified complexes. (D) Time-lapse fluorescence images of Rpn8-GFP in wild-type (top) and imp1Δ (bottom) cells. Arrows indicate Rpn8-GFP midzone localization in wild-type cells. Bars, 5 µm. Graphs represent mean and standard deviation. n is the total number of cells scored from three independent experiments.