Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Nonreducing 12.5% SDS-PAGE gel of selected monomeric refolded recombinant His-tagged IFNγR1 variants. Proteins were extracted from inclusion bodies by 8 M urea, further purified on Ni-NTA agarose, and dialyzed, and monomeric fraction was separated on gel filtration column (see above). IFNγR1 with C-terminal His-Tag migrates at a molecular mass of 23 kDa when analyzed on nonreducing SDS-PAGE gel.
