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Normalized melting curves of IFNγR1 variants measured by temperature-dependent near ultraviolet circular dichroism (CD) spectra. Each data point is from the intensity measured at 280 nm. IFNγR1 WT, V35L, N96W, and N96W + V35L variants were measured in PBS buffer between 25 and 65°C at steps 1°C/minute. The melting temperature (T m) of IFNγR1 variants was determined as 54°C for WT, 53°C for V35L, 50°C for N96W + V35L, and 48°C for N96W, respectively.
