Figure 4.

BRAF mutant cells outcompete parental B16tk in culture. Parental B16tk cells, infected with a lentiviral vector expressing GFP, were cocultured with (a) an explant of a B16tk primary tumor from a mouse treated with PBS; (b) with B16tk REC#1 (BRAF^G596R) cells; or (c) with B16tk REC#3 (BRAF^V600E) cells at varying ratios as shown starting at 100% B16tk:0% partner cell type (10⁵ cells total). Cultures were split 1:5 every 48 hours and 7 days later the % of B16tk parental cells (GFP +ve) was analyzed by FACS. Results representative of three separate experiments.