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. 2015 Mar 10;23(5):907–917. doi: 10.1038/mt.2015.22

Figure 7.

Figure 7

BIM activates cytochrome c release in a range of cancer cells lines with varying potency. Mitochondria isolated from 10 different cancer cell lines were incubated with BIM or BIM/LE for 30 minutes at a range of concentrations (0.01–100 µmol/l). BIM/LE (L62E) has a knockout mutation in the binding interface. The % cytochrome c release in the supernatant was quantified using an ELISA assay. The % cytochrome c release was defined as ((A450, sample)–(A450, DMSO))/((A450, Triton-X100)–(A450, DMSO)) × 100, where DMSO was used as a negative control and 1% Triton-X100 as a positive control. Data represents mean ± SD with n = 4. A nonlinear dose–response curve was fit to each data set using GraphPad Prism to determine the potency (EC50) of BIM in each cell line (italicized in the figure).