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. Author manuscript; available in PMC: 2015 May 12.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Feb 26;35(4):865–876. doi: 10.1161/ATVBAHA.114.304768

Figure 2.

Figure 2

Vascular recovery in etv2 morpholino (MO)-injected etv2:Kaede embryos is mediated by vascular endothelial cells specified before the 20-somite stage. A and B, etv2:Kaede expression in the trunk of 36-hpf embryos after photoconversion at the 20-somite stage (19 hpf). A, Control embryos developed intersegmental vessels (ISVs) which were almost exclusively both red and green indicating that cells contributing to ISVs were specified before the 20-somite stage in normally developing embryos. B, Etv2 morpholino knockdown in etv2:Kaede embryos reduced angiogenic sprouting, as expected. A majority of ISVs in etv2 morphant embryos were red+green (arrows), with a small number of green only ISVs (asterisk) observed. C, Etv2 knockdown did not cause a significant change in the number of green only ISVs. Error bars, +1SD. WT indicates wild-type.