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. Author manuscript; available in PMC: 2015 May 12.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Feb 26;35(4):865–876. doi: 10.1161/ATVBAHA.114.304768

Figure 6.

Figure 6

Apoptosis of vascular endothelial cells is expanded in double Etv2;Fli1b-deficient embryos. A-D, Whole-mount immunohistochemical staining of 48-hpf embryos for caspase 3 and green fluorescent protein (GFP) expression. Lateral views shown with anterior to the left. No distinct zones of apoptosis were noted in wild-type (A) and fli1b-/- embryos (C). By contrast, etv2 morphant embryos showed a narrow zone of apoptosis in the vascular plexus (B). D, This zone of apoptosis was expanded in embryos deficient in both fli1b and etv2 (arrows). Hbbe3 (A′-D′) and gata1 (A″-D″) levels determined by whole-mount in situ hybridization show normal erythrocyte staining in all groups indicating the observed apoptosis was not because of a loss of blood cells. Fractions indicate the number of embryos with the observed pattern of marker expression (numerator) and the total evaluated (denominator).