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. Author manuscript; available in PMC: 2015 May 12.
Published in final edited form as: J Neurochem. 2015 Mar 13;133(4):572–581. doi: 10.1111/jnc.13079

Table 1.

Expression of genes related to fructose metabolism in rat neocortex

Gene Function Neocortex mRNA by microarray Neocortex mRNA by PCR Liver mRNA by PCR
Aldose reductase Glucose → Sorbitol 730 ± 90
Sorbitol dehydrogenase Sorbitol → Fructose 170 ± 10
Ketohexokinase Fructose → Fructose-1P n.s.* 0.042 ± 0.016 638 ± 36
Hexokinase 1 Fructose → Fructose-6P 1060 ± 70 1060 ± 80** 13 ± 2
Aldolase A graphic file with name nihms680222t1.jpg Fructose-1,6P → DHAP+GA3P
or
Fructose-1P → DHAP + GA		 8920 ± 330
Aldolase B n.d.
Aldolase C 5080 ± 870
Glut5 Fructose transporter n.r.e. 43 ± 4 27 ± 5
Glut1 Glucose transporter 420 ± 50 793 ± 63 18 ± 3
Glut3 Glucose transporter 440 ± 50 1150 ± 23 1.9 ± 0.3

mRNA from frontal cortex (neocortex) of six male Wistar rats was subjected to microarray analysis (rat GeneChips, genome RA230.2, Affymetrix). The values are fluorescence intensities in arbitrary units; the mean expression value for all 16 000 detected genes was 190. Brain and liver total RNA was prepared from each of five Wistar male rats and subject to qRT-PCR. Values are normalized to the mean of the expression level for three housekeeping genes (HPRT1, β-actin and GAPDH). Data are mean ± SD values. n.s.: not significantly different from zero; n.d.: not detectable; n.r.e. not reliably expressed at 37 ± 9. DHAP: dihydroxyacetone phosphate, GA3P: glyceraldehyde-3 phosphate: GA: glyceraldehyde.

*

reliably expressed only in 3 out of 6 samples, but reliably expressed in all 7 brain stem samples at 63 ± 17.

**

For comparison with the microarray data, qRT-PCR data for the brain samples were normalized to a value of 1060 for hexokinase 1.