Table 2.
Plasmids
| Name | Details | Origin |
|---|---|---|
| pRS406 | URA3 integrating vector | [45], Euroscarf |
| pSF019 | TEF1 promoter containing vector | [29] |
| yEGFPCLN2PEST-pRS406 | Destabilized GFP base plasmid | [36] |
| PMAL12- yEGFPCLN2PEST-pRS406 | MAL12 promoter driven GFP expression | This study |
| PGLC3- yEGFPCLN2PEST-pRS406 | GLC3 promoter driven GFP expression | This study |
| PGPH1- yEGFPCLN2PEST-pRS406 | GPH1 promoter driven GFP expression | This study |
| PSUC2- yEGFPCLN2PEST-pRS406 | SUC2 promoter driven GFP expression | This study |
| PTEF1-yEGFPCLN2PEST-406 | Constitutive TEF1 promoter driven GFP expression | This study |
| pRS413 | Yeast centromeric plasmid with HIS3 selection marker | [45], Euroscarf |
| CYC1t-pRS413 | CYC1 terminator | This study |
| PSUC2-CYC1t-pRS413 | SUC2 promoter, CYC1 terminator | This study |
| PSUC2-GFPantisense-CYC1t-pRS413 | SUC2 regulated expression of an antisense RNAi construct for GFP | This study |
| pRS404-PTEF -Ago1-CYC1t | TRP1 integrating vector with constitutive Argonaute expression | [5] |
| pRS405-PTEF-Dcr1-CYC1t | LEU2 integrating vector with constitutive Dicer expression | [5] |
| pUG6 | LoxP-KanMX-LoxP cassette | Euroscarf |
| pUG66 | LoxP-Ble-LoxP cassette | Euroscarf |