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. 2015 May 6;16(1):54. doi: 10.1186/s12931-015-0214-6

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© Sheridan et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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p65 expression and localization in Normal, At Risk and COPD lung fibroblasts. (A) p65 expression: There was no perceptible difference in p65 expression between Normal, At Risk and COPD-derived lung fibroblasts exposed to 2% CSE for up to 24 hours. Representative western blot is shown of 3 independent experiments. (B) p65 localization: There was little perceptible difference in the localization of p65 in lung fibroblasts from the three subject groups, which appeared predominantly cytoplasmic (red colour). Exposure to 2% CSE for 30 minutes did not appreciably alter the localization of p65. Representative images shown are based on two independent experiments.