Table 2.
Results of the pathogenicity and by contact transmission experiment
| Virus | Sample | Day pi/pc | Infected | Sentinels | ||||
|---|---|---|---|---|---|---|---|---|
| RRT-PCR | Ct ± SD | VI | RRT-PCR | Ct | VI | |||
| Os/H7N1 | Lungs | 3 | +2/2 | 26.22 ± 1.1 | +2/2 | - | - | nt |
| 4 | +2/2 | 22.33 ± 0.08 | +2/2 | +1/2 | 28.24 | +1/1 | ||
| Nasal wash | 3 | +1/2 | 32.13 | - | - | - | nt | |
| 4 | +1/2 | 30.57 | - | +1/2 | 35 | - | ||
| Brain | 3 | +1/2 | 30.01 | - | - | - | nt | |
| 4 | - | - | nt | - | - | nt | ||
| Spleen | 3 | +2/2 | 28 ± 3.5 | +1/2 | - | - | nt | |
| 4 | - | - | nt | - | - | nt | ||
| Kidney | 3 | +1/2 | 29.6 | - | - | - | nt | |
| Tk/H5N1 | Lungs | 2a | +1/1 | 20.22 | +1/1 | nt | nt | nt |
| 3 | +2/2 | 22.19 ± 2 | +2/2 | - | - | nt | ||
| 4 | +2/2 | 27.3 ± 3.4 | +2/2 | +1/2 | 24.03 | +1/1 | ||
| Nasal wash | 2a | +1/1 | 26.95 | - | nt | nt | nt | |
| 3 | +2/2 | 29 ± 2.1 | +2/2 | - | - | nt | ||
| 4 | +2/2 | 34.7 ± 0.1 | - | - | - | nt | ||
| Brain | 2a | - | - | nt | nt | nt | nt | |
| 3 | - | - | nt | - | - | nt | ||
| 4 | +1/2 | 33.48 | - | - | - | nt | ||
| Spleen | 2a | +1/1 | 33.12 | - | nt | nt | nt | |
| 3 | - | - | nt | - | - | nt | ||
| 4 | - | - | nt | - | - | nt | ||
pi: post-infection; pc: post-contact; Ct: quantification cycle; SD: standard deviation; VI: virus isolation; adead vole with clinical signs on day 2 pi; −: all samples tested negative; nt: not tested.
RRT-PCR (mean of Ct values ± SD) and virus isolation results of the different organs and nasal washes collected from infected voles and in contact sentinels on days 3 and 4 pi/pc.