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. 2015 Mar 23;16(5):654–669. doi: 10.15252/embr.201439939

Figure 4. ETV2 is required for the generation and expansion of hemangioblast.

Figure 4

  • A Etv2+/+ and Etv2−/− yolk sac and embryo proper were analyzed for FLK1 and PDGFRα expression at E7.5, 8, and 8.5 (n = 6 for each). Representative FACS analysis at each time point is shown.
  • B Gross morphology of the E10.5 Flk1Cre;Etv2 CKO embryos is shown. Note the wrinkled yolk sac (red arrow) and the swollen pericardial cavity accumulated with fluid/blood (yellow arrow) in the mutants.
  • C FACS analysis of E8.5 Flk1Cre;Etv2 CKO embryos for FLK1 and PDGFRα expression.
  • D CD31/PECAM1 whole-mount staining of the E9.5 embryos (up: embryos; down: yolk sacs).
  • E Hematopoietic colony assay of the E8.5 wild-type and mutant yolk sacs. Mean ± SD, n = 4 biological replicates. ***P < 0.001, two-tailed Student's t-test.
  • F, G Microarray analysis (F) and qRT–PCR analysis (G) of the Flk1Cre;Etv2 CKO yolk sacs. Mean ± SD, n = 3 biological replicates. *P < 0.05, ***P < 0.001, two-tailed Student's t-test.