A–C A representative FACS profile of FLK1 and PDGFRα expression of E8.5 Fli1+/+ (n = 9), Fli1+/− (n = 11), and Fli1−/− (n = 6) embryos (A) (embryos were obtained from 4 to 5 pregnant mice); Fli1+/+ and Fli1−/− EBs (B, four independent biological samples); and iFli1 EBs (± DOX) (C, four independent biological samples) at indicated times.
D iEtv2-GS (=iEtv2-2A-Gata2-2A-Scl), iFli1-GS, iErg-GS, and iEts1-GS EBs were treated with DOX from day 2 and analyzed for FLK1 and PDGFRα at indicated times. Numbers indicate the percentage within each quadrant. Representative results from four independent biological samples are shown.
E The enrichment of ETV2 and FLI1 binding at the indicated gene loci by ChIP-qPCR analysis in wild-type EBs is shown. qPCR primers and genomic locations are provided in Supplementary Table S3. Error bars represent SD, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001 by Student's two-tailed t-test.
F A model showing ETV2 regulation of the hematopoietic and endothelial cell program.
