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. 2015 May 12;6:329. doi: 10.3389/fpls.2015.00329

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Copyright © 2015 Abeynayake, Etzerodt, Jonavičienė, Byrne, Asp and Boelt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential expression of fructan-related genes in the green tissues (top) and root tissues of perennial ryegrass (Lolium perenne L.) variety “Veyo” and ecotype “Falster” during cold acclimation. (A,B) Sucrose: sucrose 1-fructosyltransferase (Lp1-SST). (C,D) Fructan: fructan 1-fructosyltransferase (Lp1-FFT). (E,F) Fructan: fructan 6G-fructosyltransferase (Lp6G-FFT). (G,H) Fructan exohydrolase (Lp1-FEH). (I,J) Fructan exohydrolase (Lp6-FEH). Changes in mRNA levels of the genes in the top and root tissues of “Veyo” (A,C,E,G,I) and “Falster” (B,D,F,H,J) are shown. Relative gene expression was measured by quantitative RT-PCR. Expression values were normalized by geometric averaging of elongation factor 1-alpha (LpEF1a), actin (LpACT11), and eukaryotic initiation factor 4A (LpeIF4a) internal control genes. Data represent the mean ± SE, obtained from three biological replicates of the analysis.