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. 2015 May 12;6:335. doi: 10.3389/fpls.2015.00335

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Copyright © 2015 Mgcina, Dubery and Piater.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization and viability of mesophyll protoplasts isolated from Arabidopsis leaves. (A) Micrograph showing protoplasts at 100X magnification (micrographs were taken to follow the digestion process prior to washing and hence debris is still visible), and (B) histogram illustrating viability over time using the FDA stain. Dark gray bars represent total protoplasts, while light gray bars represent viable protoplasts, with error bars indicating the standard deviation of three independent biological repeats relative the control set at 100%.