FIGURE 7.

Graphs illustrating binding reversibility of LPS-conjugates to mesophyll Arabidopsis binding sites. Protoplasts were pre-incubated for 30 min with different concentrations of unlabeled LPS_{E. coli}, as indicated, followed by treatment with (A) 0.4 μg/ml Alexa-LPS_{E. coli} and (B) 0.46 μg/ml Qdot-LPS_{E. coli}. Sole treatment (without unlabeled LPS pre-incubation) with labeled LPS-conjugates also served as positive controls. The negative controls represent unlabeled LPS_{E. coli}-treated Arabidopsis mesophyll protoplasts set at 100%, with error bars representing standard deviation of three independent biological repeats.