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. 2015 May 12;6:335. doi: 10.3389/fpls.2015.00335

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Copyright © 2015 Mgcina, Dubery and Piater.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The role of exo- (BFA) and endocytosis (Wortmannin) inhibitors in protoplasts treated with labeled LPS-conjugates. Samples were co-treated simultaneously with either inhibitor and (A) 0.4 μg/ml Alexa-LPS_{E. coli} and (B) 0.46 μg/ml Qdot-LPS_{E. coli} respectively, followed by incubation at RT for 120 min. Sole treatment with each LPS-conjugate served a positive control and set to 100%, while BFA and Wortmannin sole treatments were included as negative controls. Error bars represent the standard deviation of three independent biological repeats.