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. 2015 May 12;9:165. doi: 10.3389/fncel.2015.00165

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Copyright © 2015 Zhang, Chen, Ni, Guo, Lu, Liu, Li, Sun, Wang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry-isolated Lgr6-positive cells can generate hair cells in vitro. (A) Lgr6-GFP-positive and Lgr6-GFP-negative cells were isolated using flow cytometry. (B) Quantitative PCR showed that isolated Lgr6-EGFP-positive cells had significantly higher Lgr6 expression, slightly higher Sox2 expression, and significantly lower Brn3.1 expression compared to the Lgr6-EGFP-negative cells. (C) Isolated Lgr6-EGFP-positive cells generated significantly more myosin 7a-positive cells than Lgr6-EGFP-negative cells. (D) Immunostaining showed that among the hair cells that developed from isolated Lgr6-EGFP-positive cells, 95% were Lgr6-EGFP-positive, 96% were Sox2-positive, and none were myosin 7a-positive. (E) Isolated Lgr6-GFP-positive cells can generate myosin 7a+ hair cells after 10 days of in vitro culture. *p < 0.05, ***p < 0.001.