Figure 9. MPYS expression in CD11C+ cells is required for the optimal mucosal adjuvant activity of CDG.
(A) Tmem173Flox/Flox, ItgaxCreTmem173Flox/Flox or Tmem173−/− mice were intranasally administered OVA (20 μg) alone or together with 5 μg CDG as in Figure 1A. Anti-OVA IgG1, IgG2C and IgA were determined by ELISA. n = 3. (B) Tmem173Flox/Flox, ItgaxCreTmem173Flox/Flox or Tmem173−/− mice were immunized with PspA (2 μg) alone or together with 5 μg CDG as in Figure 1C. Anti-PspA IgG1, IgG2C, and IgA were measured by ELISA. n = 3. (C–D) Splenocytes and lung cells from PspA or CDG + PspA immunized Tmem173Flox/Flox, ItgaxCreTmem173Flox/Flox or Tmem173−/− mice were stimulated with 5 μg/ml PspA for 4 days in culture. Cytokines were measured in the supernatant by ELISA as in Figure 1D n = 3. (E) 1 month after the last immunization, CDG/PspA or PspA immunized Tmem173Flox/Flox and ItgaxCreTmem173Flox/Flox mice were infected (i.n.) with S. pneumoniae (D39 strain, ∼5.0 × 106 c.f.u.). At 48 hr post infection, lung bacterial burden were determined. n = 2. Graph present means ± standard error from three independent experiments. Significance is represented by an asterisk, where p < 0.05.
Figure 9—figure supplement 1. The impaired CDG response in ItgaxCreTmem173Flox/Flox mice is not due to the over-expression of Cre in CD11C+ cells.
