. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: J Cell Biochem. 2014 Oct;115(10):1637–1645. doi: 10.1002/jcb.24834

TABLE 1.

Examples of FACS Strategies Used to Isolate Basal Cell Progenitors

Sorting strategy	Assay type	Refs.
Mouse
ITGAS; p63; NGFR; Krt5	3D sphere-forming assay	Rock et al. [2009]
EpCAM^high ;ITGA6; CD104; CD24^low	Organoid-forming co-culture assay	McQualter et al. [2010]
ITGA6^highSca-1; ALDH	Irradiated fibroblast co-cultures	Ghosh et al. [2011b]
H2B-GFP^{high a}; [TGA6-Sca-1	Irradiated fibroblast co-cultures	Ghosh et al. [2013b]
TROP₂; ITGA6; ALDH^high	3D sphere-forming assay	Hegabet al. [2014]
Human
TF;CD151	ALI culture, xenograft assay	Hajj et al. [2006]
Hoescht 33342^low; CD45^neg	All culture	Hackett et al. [2003]
CD44;CD166;ALDH^high	3D sphere-forming assay	Hegab et al. [2012]
TF; CD151^neg	Irradiated fibroblast co-cultures	Ghosh et al. [2013a]

H2B-GFP retention indicated infrequent in vivo proliferation while dilution indicated frequent division.