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. 2015 May 12;6:448. doi: 10.3389/fmicb.2015.00448

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Copyright © 2015 Woldringh, Hansen, Vischer and Atlung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cells of E. coli FH4035, grown in glycerol minimal medium at 28°C, were treated during 100 min with the indicated inhibitors, fixed with 0.1% OsO4 and stained with 10 μg/ml DAPI. (A) In the control cells divided and well separated nucleoids occur in 15% of the cells. (B) In rifampicin-treated cells only 9% of the cells show divided nucleoids. (C) In chloramphenicol-treated cells the nucleoids (only 3% divided) have a more spherical shape.