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. 2015 May 7;13:143. doi: 10.1186/s12967-015-0494-0

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© Conforti et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vitro immunoregulatory properties of HD- and Cys-MSCs. A: Immunophenotype of culture-expanded Cys-MSCs. MSCs are positive for CD90 and CD13 surface antigens and negative for CD34 and CD14 molecules. B: In vitro immunomodulatory effect of HD-MSCs and Cys-MSCs on peripheral blood mononuclear cells (PBMCs) in an allogeneic setting. The graph shows the mean percentage of proliferation of PBMCs stimulated with phytohemagglutinin (PHA) in the absence or in the presence of HD-MSCs or Cys-MSCs at different MSC:PBMC ratios (1:2 and 1:20), calculated by measuring ³H-thymidine incorporation after 3-day co-culture. All experiments were performed in triplicates in an allogeneic setting (i.e. Cys-MSCs/HD-PBMCs).