Figure 3.

Expression of bpa-miR-5364 is upregulated following host invasion and in cultured larvae. A. qRT-PCR confirms bpa-miR-5364 is up-regulated between mosquito-derived L3 and 24 h p.i. L3. Levels of bpa-miR-5364 were assessed relative to the constitutively expressed bpa-mir-100c and are expressed as log₂fold change relative to mosquito-derived (MD) L3. The mean of three biological replicates is shown +/− standard deviation; replicates were independent from those used in the microarray analysis. B. bpa-miR-5364 expression determined by qRT-PCR in B. pahangi L3 cultured at 37°C in worm culture medium that was either FCS-free, or contained 5% or 10% FCS. Levels of bpa-miR-5364 were normalised to bpa-miR-100c and are expressed as log₂fold change relative to mosquito-derived (MD) L3. The mean of three technical replicates from a single biological replicate is shown +/− standard deviation.