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. 2015 May 12;5:9685. doi: 10.1038/srep09685

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(a) Multicopy overexpression of S. cerevisiae PMP3 (ScPMP3) and its homologs from C. glabrata (CgPMP3) and C. albicans (CaPMP3-O: PMP3 ortholog, orf19.1655.3; CaPMP3-B: PMP3 best hit, orf19.2959.1) in pmp3Δ strain of S. cerevisiae enhance AmB resistance by about 4-fold with respect to wild-type strain (BY4741) and about 32-fold with respect to pmp3Δ strain. The relative growth of the strains on 0.1 μg/ml AmB (not shown) was comparable to that of respective strains on 0.2 μg/ml AmB. (b) AmB sensitivity of C. glabrata strain deleted in PMP3 ortholog (Cgpmp3Δ) and C. albicans strains deleted in both alleles of PMP3 ortholog (Capmp3-OΔ/Δ) and PMP3 best hit (Capmp3-BΔ/Δ), with respect to their respective parent strains CG462 and SN95. Five μl of 10-fold serial dilutions of cells were spotted starting from about 10⁵ cells/spot, as described in Methods.