Table 1.
PTX, LEV-PTX, 4AB-PTX, individual and mixed polymeric micelles cytotoxicity in MCF-7 and SK-OV-3 human cell lines. Cells were incubated with the each formulation for 72hr thereafter cell viability was assessed by CellTiter-Blue® Cell Viability Assay.
| Formulation | a IC50 (nM) (Mean ± SD) | |
|---|---|---|
| MCF-7 | SK-OV-3 | |
| PTX | 4.60 ± 1.17 | 1.84 ± 0.40 |
| LEV-PTX | 11.17 ± 0.66 | 5.17 ± 2.18 |
| 4AB-PTX | 17.19 ± 1.05 | 29.02 ± 5.06 |
| PEG-p(Asp-Hyd-LEV-PTX) (individual micelle) | 8.00 ± 0.614 | 4.43 ± 0.86 |
| PEG-p(Asp-Hyd-4AB-PTX) (individual micelle) | --- | --- |
| Mixed Micelle 1:1 | 12.58 ± 3.25 | 6.21 ± 1.64 |
| Mixed Micelle 1:5 | 9.72 ± 2.28 | 8.72 ± 0.99 |
a
IC50 was calculated by fitting the data to log(inhibitor) vs. response -Variable slope equation using GraphPad Prism version 5.00 for Windows. (Mean ± SD, n=3)