Table 2.
Cellular parameters of wt and tol-pal mutant cells.
Straina | TB28/pTB6 | FB20229/pTB6 | MG5/pTB6 | MG4/pTB6 | FB20229/pNP4 | MG5/pMG36 |
---|---|---|---|---|---|---|
Genotype | wt/Plac∷TTgfp | tolA/Plac∷TTgfp | pal/Plac∷TTgfp | tolQ-pal/Plac∷TTgfp | tolA/Plac∷gfp-tolA | pal/Plac∷pal-rfp |
Number of cells | 343 | 381 | 230 | 290 | 367 | 266 |
Width (SD) in μm | 1.33 (0.08) | 1.64 (0.11) | 1.58 (0.10) | 1.57 (0.12) | 1.42 (0.09) | 1.40 (0.08) |
Length (SD) in μm | 3.51 (0.73) | 3.36 (0.85) | 3.12 (0.68) | 3.25 (0.73) | 3.60 (0.73) | 4.24 (0.94) |
Length/width | 2.64 | 2.05 | 1.98 | 2.07 | 2.53 | 3.09 |
% cells with single constr. | 43 | 60 | 86 | 68 | 44 | 78 |
Constriction position (SD) | 0.49 (0.03) | 0.49 (0.03) | 0.48 (0.03) | 0.48 (0.03) | 0.48 (0.02) | 0.49 (0.03) |
% cells w. multiple constr. | 0 | 3 | 1 | 1 | 0 | 0 |
Number of OM blebs | 0 | 207 | 246 | 218 | ND | 2 |
% cells with OM blebs | 0 | 44 | 81 | 54 | ND | <1 |
% of blebs at constr. site | NA | 42 | 31 | 37 | ND | NA |
% of blebs at polar caps | NA | 53 | 64 | 56 | ND | NA |
% of blebs elsewhere | NA | 5 | 5 | 7 | ND | NA |