. Author manuscript; available in PMC: 2015 May 12.

Published in final edited form as: Nat Protoc. 2014 Jan 2;9(1):209–222. doi: 10.1038/nprot.2014.005

TABLE 3.

Sample CD8αβ T cell ex vivo staining panel.

Fluorochrome	Marker	Ab clone	Company	Dilution
PE	CD44	IM7	eBioscience	2 µg ml⁻¹ (1:100)
PerCP-Cy5.5	CD8P	YTS156.7.7	BioLegend	2 µg ml⁻¹ (1:100)
APC-eF780	CD62L	MEL-14	eBioscience	2 µg ml⁻¹ (1:100)
Aqua	LIVE/DEAD	-	Invitrogen	Per protocol
APC	CD8α i.v.	53–6.7	eBioscience	-