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. 2014 Oct 28;1:20. doi: 10.3389/fmolb.2014.00020

Figure 4.

Figure 4

Small angle X-ray scattering results for the endophilin-A1-BAR complex with arachidonyl-CoA micelles. (A) Scattering profiles of free endophilin-A1-BAR at c = 2 mg/ml (1), arachidonyl-CoA micelles at c = 1 mg/ml (2) and their complex at c = 3.5 mg/ml (3). Experimental data are denoted by black dots, fits from the ab initio and atomic structure-based models are shown as red solid and blue dashed lines, respectively. The curves are displaced in logarithmic scale for better visualization. (B) Structural models. Top panel: ab initio DAMMIN model of the arachidonyl-CoA micelle (left) and the one built from the atomic structure of monomers using icosahedral symmetry (right). Middle panel: ab initio DAMMIN model of the complex (gray beads) overlaid with rigid body model (endophilin is shown as blue backbone, two arachidonyl-CoA micelles are shown in red). Bottom panel: Multiphase bead model of the complex reconstructed by MONSA by simultaneous fitting of two scattering patterns. (C) The scattering profile of endophilin-A1-BAR at 4 mg/ml (top) changes upon titration of increasing concentrations of arachidonyl-CoA (0, 1, 2, 3, 4, 6, 8 mg/ml, from top to bottom).