Figure 1.

Reovirus administered i.v. is rapidly neutralized by Nab, but functional virus can be found associated with CD11b⁺ cells. (a) C57Bl/6 mice were injected with 10⁷ TCID₅₀ reovirus i.v. 2, 30, or 60 minutes later mice (three mice per time point); blood was collected by cardiac puncture and reovirus titer was determined by plaque assay. (b) Thirty minutes after i.v. injection of 10⁷ TCID₅₀ reovirus, the intact packed cell fraction was harvested and reovirus titer was determined by plaque assay. In separate mice, the CD11b⁺ and CD11b⁻ fractions were recovered by microbead separation and titered for reovirus.