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. 2014 Aug 5;22(10):1730–1740. doi: 10.1038/mt.2014.125

Figure 1.

Figure 1

Generation of transgenic EcN expressing AvCys. (a) Polymerase chain reaction with AvCys specific primers amplified the AvCys gene in EcN-AvCys (2) and positive control (4), but not in EcN (1) or the negative water control (3). (b) Western blot analyses using an AvCys-specific antibody on supernatants of EcN (1) and EcN-AvCys (2) grown in Luria broth medium and recombinant AvCys as positive control (3). (c) Western blot of 1 ml of an Iscove's modified Dulbecco medium (IMDM) control (1), supernatant from EcN-AvCys grown in IMDM (2), supernatant from EcN grown in IMDM (3), and 7 lanes loaded with recombinant AvCys as a standard ranging from 200–5 ng (4).