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. 2014 Aug 5;22(10):1730–1740. doi: 10.1038/mt.2014.125

Figure 4.

Figure 4

Effects of EcN-AvCys on local cytokine production and Tregs in DSS colitis. (a) Concentration of IL-17A in supernatants of mLN cells stimulated with concanavalin A for 48 hours as determined by ELISA. (b) Frequencies of Foxp3+ Tregs in the CD4+ population in mLN as detected by flow cytometry. (c,d) Levels of IL-6 (c) and active TGF-β in supernatants of distal colon explants cultured for 24 hours expressed in relation to weight of cultured tissue. Data in (a) and (b) are pooled from three individual experiments with 2–3 (naïve controls)—5 mice (DSS, DSS + probiotics) per group. Mean + SEM is shown. Data in (c) and (d) depict analyses of two colon explants derived from individual mice (n = 3–5 per group) and mean ± SEM is shown. Data derive from one of two individual experiments with similar results. *P < 0.05, **P < 0.01, ***P < 0.001 (Mann–Whitney U-test).