Figure 3.

Aminoglycosides increase COL7A1 mRNA levels in recessive dystrophic epidermolysis bullosa (RDEB) keratinocytes. mRNAs were isolated from normal immortalized human keratinocytes (IKC) or RDEB1 and RDEB2 keratinocytes either untreated or treated with G418 (8 µg/ml) or gentamicin (400 µg/ml) for 24 hours and then subjected to real-time RT-PCR. The relative amounts of C7 mRNA levels compared with β-actin mRNA levels are shown as means + SD from two independent experiments. In each experiment, samples were run in triplicate.