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. 2014 Aug 26;22(10):1741–1752. doi: 10.1038/mt.2014.140

Figure 6.

Figure 6

Aminoglycoside-induced C7 incorporates into the dermal-epidermal junction (DEJ) of in vitro skin equivalents. The cyrosections were subjected to immunofluorescent labeling using a monoclonal antibody (NP185) specific to human C7. Left panels are skin equivalents composed of untreated recessive dystrophic epidermolysis bullosa (RDEB) keratinocytes (RDEB1 or RDEB2) combined with untreated RDEB fibroblasts (RDEB4). Middle panels are skin equivalents composed of RDEB keratinocytes (RDEB1 or RDEB2) treated with gentamicin (400 μg/ml) combined with RDEB fibroblasts (RDEB4). Right panels are skin equivalents composed of normal human fibroblasts combined with normal human keratinocytes. d, dermis; e, epidermis. Arrows indicate DEJ. We have performed three independent experiments and similar results were obtained.