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. 2015 Feb 5;2:2. doi: 10.3389/fmolb.2015.00002

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Copyright © 2015 Faggiano, Menon, Kelly, Todi, Scaglione, Konarev, Svergun, Paulson and Pastore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mapping ubiquitination on the Josephin surface. (A) Chemical shift perturbation of the changes occurring on ¹⁵N labeled Josephin domain after ubiquitination of JosK117-only. (B) Far-UV CD spectra of JosK117-only (blue), mono-ubiquitinated JosK117-only (green), ubiquitin (yellow). The arithmetical sum of the spectra of JosK117-only and of free ubiquitin is reported in dashed cyan. (C) Cross-saturation experiment on perdeuterated ¹⁵N JosK117-only covalently attached to unlabeled ubiquitin on K117. (D) ¹⁵N HSQC spectra for the C14 peak before (blue) and after (green) ubiquitination.