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. 2015 Feb 5;2:2. doi: 10.3389/fmolb.2015.00002

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Copyright © 2015 Faggiano, Menon, Kelly, Todi, Scaglione, Konarev, Svergun, Paulson and Pastore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of ubiquitin on the enzymatic kinetics. (A) Comparison of the cleavage rate of ubiquitin-AMC (expressed in relative fluorescence units per minute) by wild-type Josephin, JosK117-only, and mono-ubiquitinated JosK117-only. (B) Comparison of the cleavage rate of JosK117-only and mono-ubiquitinated JosK117-only with that of another typical DUB, isopeptidase T, at a concentration 10 times lower. (C,D) Enzymatic activity respectively of JosK117-only and mono-ubiquitinated JosK117-only in the presence of increasing concentrations of free ubiquitin.